A Certificate of Analysis, or COA, is the laboratory report for one specific production batch. It records identity, purity, net content, and endotoxin results for that lot, so the material in a given vial can be traced back to the assay data that describes it.
Which laboratory performs the testing?
Testing is performed by Freedom Diagnostics, an independent third-party analytical laboratory. Purity and identity are determined by HPLC coupled with mass spectrometry, and endotoxin is screened as a separate assay. The signed reports are published on the Lab Reports page.
How do I match a vial to its certificate?
Locate the accession or batch number printed on the vial label, enter it in the search box on this page, and open the matching signed report. The batch number on the label and the batch number on the certificate should be identical.
What does a 99%+ HPLC purity result actually measure?
HPLC separates a sample into its components and reports the target peptide as a percentage of total detected peak area. A 99%+ result means the named peptide accounts for at least 99% of the material detected in that batch, and mass spectrometry confirms the identity of that peak.
What a certificate of analysis records, field by field
A certificate is a record of one laboratory event: a named sample, drawn from a named production run, run through a named assay, on a named date. Each field on the report exists to make that chain checkable. The table below describes what every field carries and why it matters when a certificate is being matched against a vial.
Fields that appear on a batch certificate and what each one establishes.
Field
What it records
Why it matters
Compound name
The peptide the sample was submitted as.
The assay result is only meaningful against a stated identity. The name on the report and the name on the label have to agree.
Accession number
The testing laboratory's own reference for the sample it received.
It is the key that lets a report be looked up against the laboratory's records rather than taken on trust from the seller.
Batch or lot number
The production run the tested sample was drawn from.
This is the number that must match the vial. A certificate for a different lot describes different material.
Date issued
When the laboratory released the signed report.
It fixes the analysis in time and shows how recent the data is relative to the material being supplied.
Test performed
Which assay produced the result, such as purity analysis or endotoxin screening.
Purity and endotoxin are separate determinations, so one batch can be documented by more than one certificate.
Method and instrumentation
The separation and detection technique used to generate the result.
The method sets the boundary of what the reported number can mean. A figure without a method behind it is not interpretable.
Result
The reported figure or the pass or fail outcome for that assay.
A purity percentage and a pass or fail outcome answer different questions and are not interchangeable.
Net content
The amount of material stated for the container the lot fills.
Purity is a proportion, not a quantity. Net content is the separate statement of how much material is present.
Analyst signature
The chemist who reviewed and released the report.
A signed report is attributable to a named person at a named facility rather than to an anonymous document.
Testing laboratory
The facility that ran the assay.
Independent testing means the facility running the assay is not the party selling the material.
Reading a purity figure correctly
A chromatographic purity figure is an area percentage. The instrument separates the sample over time, the detector responds to what elutes, and the target peak is expressed as a share of the total detected peak area. That is a relative measurement, not an absolute mass balance. Anything the detector does not respond to under the conditions used, such as residual solvent, water content, or counter-ion salt, is not represented in that percentage and is established by separate determinations when it is reported at all.
Identity, purity, and endotoxin are three different questions
Identity asks whether the material is the compound named on the label. Purity asks what proportion of the detected material that compound represents. Endotoxin screening asks whether bacterial endotoxin in the batch falls within a compendial limit. A batch can answer one of those questions well and still need the other two answered on their own certificates, which is why a complete record for a lot is sometimes a set of reports rather than a single page.
Reference material for laboratory research use only. Certificates describe the analysis of a submitted sample and are not a statement of suitability for any human or veterinary purpose.
